dextran hydrodynamic radius

Harding, S. E.; Varum, K.; Stoke B.; Smidsrod, O. Synthesis of water-soluble and water-insoluble amphiphilic derivatives of dextran in organic medium. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. Thus, it is accepted that, for every flexible-chain polymer in a (ideal) solvent, there is a universal value of 0 = 2.1-2.5x1023mol-1 [52]. Journal of Chemical Education, 49(8), 577-579. The micropore formed at the SC through to the epidermis, enabling drug delivery. 2018 Aug 9;23(8):1984. doi: 10.3390/molecules23081984. Pure Appl. Pathan IB, Setty CM. Bahary, W.S. Barshtein, G.; Tamir, I.; Yedgar, S. (1998). The enhanced permeability through micropores was confirmed by Franz diffusion cell. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. Degrees of long-chain branching in dextrans. Dextrans can function efficiently as anterograde or retrograde tracers, Journal Argentine Chemical Society 100 (2013) 25-34. Walkenhorst, S.; Olivier, R. (2002). Bahary, W. S.; Hogan, M. P.; Jilani, M.; Aronson, M. P. (1995). The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. Peptide and protein transdermal drug delivery. The genus Weissella: taxonomy, ecology and biotechnological potential. The Mark-Houwink parameters and the effect of temperature on them are specifically studied. WebThe study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. Skin barrier disruption by acetone: observations in a hairless mouse skin model. Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. Carbohydrate Polymers, 79, 380-390. Beer, M.U. Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! There is a deviation from linearity after 580kDa [69, 70]. Im Tiefkhlgert (-5 bis -30C) lagern und vor Licht schtzen. Polymers | Free Full-Text | Confinement-Induced Fractionation and Kirkland, J. J.; Dilks, C. H.; Rementer, S. W. (1992). After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining. Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. dextran Recent Pat Drug Deliv Formul. Viscosity, sedimentation, and light-scattering properties of fraction of an acidhydrolyzed dextran. 2005;22(4):5505. Therefore, the trend of "a" and kM-H decreases with T, where the presence of ethylene glycol or dimethylsulfoxide strongly changes the interactions of this solvent with the macromolecule. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. HHS Vulnerability Disclosure, Help Saudi Pharm J. The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. The above data are not useful because it can not be compared with those obtained in this work. Although we didnt conduct assay for inflammation, we could not find any inflammation symptoms such as redness, blisters, cracked, and thickening. Manage cookies/Do not sell my data we use in the preference centre. (2012). Proc. DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. Alternatives to animal testing: a review. Use of the single point method is correct because it is based in the Huggins method (HM); owing the HM requiring many viscosity and density measurements (at least four) for each Mw, but Solomon Ciuta method with a single measure is sufficient. However, when molecules deviate from globular to non-spherical or elongated structures then Rg/Rh tend to values upwards of Pharmaceut Sci Tech. 4K and M.W. This result could reflect the presence of some aggregated species within the lysozyme sample solution. 56 The linkage that the enzyme dextranase breaks down is indicated by the black arrow. IV. Solution properties of branched dextran. The proportionality between []0 and M0.5 is obtained as a limiting law for the no draining regime. Molecular weight determination of polysaccharides. Thermo Fisher Scientific. By using this website, you agree to our In summary, we demonstrated effective enhancement of skin permeability and delivery of macromolecules lower than 20KM.W., and regeneration of human cadaver skin after microporation with an RF-based microporation device. Microporation techniques for enhanced delivery of therapeutic agents. A few percent of R (13) glucosidic linkages provides side chains which appear to be short. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. Figure 2 shows the classical method for calculating Mark-Houwink parameters, where a linear relation between the intrinsic viscosity and the molecular weight is established for each temperature. Dextran standards with sequence molecular weight from 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa were utilized for the plot of calibration curve. Small Intestinal Permeability and Gut-Transit Time Determined Hydrodynamic properties, such as the intrinsic viscosity, [], and equilibrium solution properties such as the hydrodynamic radius RH can be combined to construct dimensionless quantities that are universal since they are of being independent of the macromolecular particle size, while they depend more or less sensitively on its shape [51]. Pharmacol Pharm. HSE and DK helped to porate the skin and to measure TEWL. Microneedles and their applications. Theory, Estimation, Experiment, and Data. This study demonstrated that hydrodynamic volume and radius of gyration of a dextran sample with M w < 20 kDa and its linear counterpart with equal M w are almost WebDextran produced from Weissella cibaria JAG8 was purified and characterized. Progr. Fluorescent dextran Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1), Microporations were performed 2, 5, and 10 times and the skin damage was confirmed using the TEWL measurement. American Journal of Food Science and Technology 2013, 1(3), 60-66. Hindered diffusion of dextran and ficoll in microporous membranes. J. Braz. The Intrinsic Viscosity of Biological Macromolecules. Table 2 compares the properties of nanoparticles coated with one or other polymer. 2023 Feb 17;12(4):861. doi: 10.3390/foods12040861. Keratinocytes adjacent to the wound site induced by the microporation in the epidermis undergo a series of modifications that allow their proliferation and migration to the wound. Ionic Polymers Based on Dextran: Hydrodynamic Properties in Aqueous Solution and Solvent Mixtures. Accueil; Solution; Tarif; PRO; Mon compte; France; Accueil; Solution; Tarif; PRO; Mon compte where tr is the column retention time in minutes (2 of 0.9792). 20K groups (n=3, *: p<0.05, **: p<0.01). Static and Dynamic Light-Scattering Solution Behavior of Pullulan and Dextran in Comparison. Results and Discussion The microscopic dynamics of proteins are probed in a light scattering experiment. J. Braz. We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Int. (2007). A novel correlation for protein diffusion coefficients based on molecular weight on radius of gyration. Comparison of single-point and dilution procedures. A similar article of dextran/water [35] clearly demonstrating the dependence of Mark-Houwink parameters with temperature. Biomaterials, 22, 1891-1898. Unable to load your collection due to an error, Unable to load your delegates due to an error. The values of Mark-Houwink parameters could be universalized with certain precautions, being an indication for the calculation of molecular weight in the temperature range of 20-40C. ; Bataille, B. US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. Based on these results a difference of approximately 1015% between the amounts of drugs permeated through the human skin and pig skin models should be considered during human clinical trials. The values of Mark-Houwink parameters universalized for the range of working temperatures are the following: 0.4267 for a*, and 0.1455 cm3/g for k*M-H, with 2 of 0.9399. The most widely used method for the characterization of macromolecules is the capillary viscometry, as it is a simple and economic method. The hydrodynamic radius (Rh) of individual samples of dextran, polyvinylpyrrolidone, and polyoxyethylene over a range of molecular weights (1,500 Journal of American Chemical Society, 76 (20), 5041-5052. 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. Huggins equation [41] gives the relation to the relative viscosity increase (i). We observe that this maximum radius is below Carbohydrate Polymers 88 1440-1444. This conjecture found to be correct. The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. An official website of the United States government. Springer Editions. SWC designed and coordinated the research. In a good solvent, which shows a zero or negative heat of mixing with the polymer, the molecule is less-extended, and the intrinsic viscosity is high. The Viscosity of Dilute Solutions of Long-Chain Molecules. The human and pig skin permeability of FITC-dextran (M.W. 20K. * shows a significant difference between the M.W. The lower molecular weight of FITC-dextran permeated more on both human and pig skin. The value of Mv is different from Mn, Mw, and Mz in a polydisperse polymer sample [38, 60, 61, 62]. AATEX. In fact, short chains do contain minor branching points. WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. The (a/b) value is 2.5 which confirms that dextran in aqueous solution is a biopolymer with a spherical conformation for an Mw range from 8.8 to 200kDa, and with tendency to compaction with increasing temperature (RH decreases). 2018;1:10915. The hydrodynamic radius is important in predicting transretinal penetration Carroll PJ. dextran dextran hydrodynamic radius. The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. (1999). Unauthorized use of these marks is strictly prohibited. The intrinsic viscosity calculation requires several concentrations, and because of this inconvenient will not use the Huggins method in this paper. Treatment of acne scars by fractional bipolar radiofrequency energy. NCI CPTC Antibody Characterization Program. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. PubMedGoogle Scholar. Cecopieri-Gmez, Martha L.; Palacios-Alquisira, Joaqun (2005). Carbohydrate Polymers, 36, 319-326. The intrinsic viscosity classical theory of random coils at conditions []0 predicts the quotient a0 = d ln[]0/d ln M to decrease from unity to 0.5 as the chain length increases. For rigid macromolecules, it is also conventional to combine a solution property with the volume of the particle itself, or with a quantity directly derived from it [54, 55, 56]. Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of. Journal of Controlled Release, 119, 5-24. Qi P, Caoa M, Songa L, Chena C, Liua M, Li N, Wua D, Penga J, Hub G, Zhao J. Seven dextrans, provided by Sigma, with molecular weight of 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa. Gold MH, Biron JA. WebFluorescein isothiocyanatedextran average mol wt 60,000-76,000; CAS Number: 60842-46-8; Synonyms: FITCDextran; find Sigma-Aldrich-FD70S MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich Unperturbed dimensions and theta temperature of dextran in aqueous solutions. Drug Deliv. The qM-H values given by equation 13, these vary from 0.9993 to 0.9841 for the range between 8.8 to 200kDa in a temperature range of 20 to 40C. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter ( dh ~ 70 nm) on order of the collagen mesh size. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. Epub 2012 Oct 18. Carbohydrate Polymers, 78, 902-907. Accessibility What is hydrodynamic radius Learn More. of different origins. Macromolecules, 33, 5730-5739. Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine Article Increased TEWL values are evidence of micropores formation. 151-166. Ahn, G.Y., Eo, HS., Kim, D. et al. Lim SB, Tingirikari JMR, Seo JS, Li L, Shim S, Seo JH, Han NS. Microporation applications for enhancing drug delivery. WebDextran 40 Pharmaceutical Quality. Macromolecules, 31, 7712-7716. dextran hydrodynamic radius. B 113, 8020-8025. European Polymer Journal, 36, 2143-2146. After 8h, the micropore became more distorted from the previous circle, suggesting that the micropores were closing. Doke SK, Dhawale SC. Conversely, fluorescein sodium was the compound selected to model hydrophilic, low MW drugs. (2003). Structural and biocompatibility properties of dextran from For 40 kDa molecules, the hydrodynamic diameter dh ~8 nm is much smaller than the collagen mesh size [ 33, 41 ]. Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. Gekko, K. (1971). WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. Surface morphology study of dextran by scanning electron microscopy showed the porous web like structure. The mechanism of the dextran-induced red blood cell Aggregation. To overcome these limitations, microporation devices that act as therapeutic vehicles for delivering drug molecules across the skin barriers have been developed. For a limited temperature range, the viscosity of a polymer solution varies generally following a relation similar to that of usual liquids (rrhenius plot), where the polymer concentration is not very high and that temperature remains far enough from glass transition [38], and for system PEAorganic solvents see reference [63]. 2013;43(4):287303. Antoniou, E.; Buitrago, C. F.; Tsianou, M.; Alexandridis, P. (2010). The hydrodynamic radius R h, defined as the radius of a hard sphere that diffuses at the same rate as the solute, takes these effects into account. Solvent effects on polysaccharide conformation. Ioan, C.E. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. government site. For a sphere and a random coil 0 the values are 9.23x1023 mol-1 and 2.60x 1023mol-1, respectively. The first term on the left-hand side of equation 2 (i/c) has a linear relation as a function of the concentration (c in g/cm3), where is the intrinsic viscosity [] is given by (c0) [42, 43]. Micropores are produced by the arrangement of microelectrodes on the skin at precise dimensions, then RF energy generated by alternating currents induces ionic vibrations between electrodes with positive or negative charges. De Belder, A.N. WebIn this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. The parameters of Mark-Houwink for polymers can vary with the polymer solution and temperature. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). European Polymer Journal, 42, 1086-1092. Huang S, Huang G. Preparation and drug delivery of dextran-drug complex. With FITC-dextran 20K, cumulative permeation in the human skin was 2.530.48M, and 3.340.19M in the pig skin, which significantly lower than FITC-dextran 4K. Cumulative amount of FITC-dextran permeated after microporation on the human and pig skin for FITC-dextran M.W.. a M.W. Hydrodynamic Stenekes, R.J.H. WebHydrodyamic Radius, R H: In addition to the analytic size of a linear (C=1) chain, the end to end distance, R eted or R 0, and the structural size, the radius or gyration, R g, dynamic measurements yield a size called the hydrodynamic radius, R H. Consider a rod particle of length L=10R where R is the radius. Drug Discov Today Technol. Webdextran hydrodynamic radius. The analysis of errors using the Solomon-Ciuta method is compared with HM [59]. Lpez Martnez, M. C.; Daz Baos, F. G.; Ortega Retuerta, A.; Garca de , J. Changes in the Mark-Houwink hydrodynamic volume of chitosan molecules in solutions of different organic acids, at different temperatures and ionic strengths. dextran hydrodynamic radius Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. Our portfolio includes for example derivatives of dextran, polysucrose, inulin and more. 2021 Aug;13(4):915-925. doi: 10.1007/s12602-021-09751-1. Durand, A. Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. There is a formula that used to calculate the radius (in Angstrom) from the molar mass (in g/mol) of dextran, a=0.33*(MM^0.46) where, a and MM are Bethesda, MD 20894, Web Policies These vibrations heat the skin tissue, triggering water vapor and cell ablation, forming microchannels from the SC through to the outer dermis. Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. A solvent at this temperature is called a theta solvent [53]. Biomater Res 24, 22 (2020). These hydrodynamic parameters show that these polysaccharides behave as a compact rigid sphere and contract by the increase of temperature (RH decreases) for the Mw range from 8.8 to 200kDa. In this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. par | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth Molecular weight (M v) and hydrodynamic properties can be calculated from there. statement and Whiteside PJD, Chininis JA, Schellenberg MW, Qian C, Hunt HK. However, considering the trauma and unethical practices associated with animal experiments, in vitro studies with human cadaver skin offers an ethical and viable alternative. (2001). Journal of Physical Chemistry. This site needs JavaScript to work properly. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Appl Biochem Biotechnol. 8600 Rockville Pike The micropores of the human cadaver skin were regenerated according to the time. ; Dutt, N.V.K. Elias, Hans-Georg (1975). 2.It can be found from the figure that at the start-up stage of the shaft, the hydrodynamic effect (HE) is weak, so the HP is not enough to support the shaft, which Pavlov, G.M. Table 2 shows that comparing the intrinsic viscosity data obtained from the Huggins method and the Solomon-Ciuta single-point method, a similar values are obtained and the %RE is lower for low Mw and it increases for higher Mw. ; Garca de , J. Tirtaatmadja, V.; Dunstan, D. E.; Boger, D. V. (2001). The skins were from the abdomen of a 39-year-old caucasian woman (BMI: 24). Chem. What is dextran? Application areas - TdB Labs AB Careers. a Microscopy images of micropore and b quantified data using ImageJ after 0 (immediately), 4, 8, and 24h according to the number of times microproration was done (n=150, *: p<0.05). The Mark-Houwink a constant is close to 0.5 in poor solvents. Cite this article. Middelkoop E, Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MMW. (2003). In the range of working temperatures and molecular weights of dextrans, similar intrinsic viscosity results obtained by other researchers and published here are slight, as shown in tables 1 and 3. Microporation was performed 2, 5, and 10 times on each sample. Dextrans in Aqueous Solution. Experimental Review on Intrinsic Would you like email updates of new search results? Viscosity of aqueous solutions of polysaccharides and hydrophobically modified polysaccharides: Application of Fedors equation. 7 8 Journal Non-Newtonian Fluid Mech., 97, 295-301. (2008). 2005;304(1):27486. It is a measure of the width of molecular weight distributions (MWD) as well as probability of molecular weight distribution curve (GPC). At the same temperature, the hydrodynamic radius and the intrinsic viscosity are higher for different molecular weights, while these properties decrease with an increase of temperature at the same molecular weight (Figure 1). Hrabchak C, Flynn L, Woodhouse KA. If plotted for all molecular weights, the double logarithm of [] and Mw, the value of a obtained in all cases shows a value smaller than 0.5, which reports that dextran macromolecule has a degree of hyperbranched with increasing molecular weight and increases much for higher Mw (< 200kDa) which making the application of M-H method more difficult.

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